Effect Of Hydroxyurea On Thrombospondin-1 And Soluble L-Selectin Levels Among Steady State Patients With Sickle Cell Anaemia

Authors

  • A. Adebayo Department of Haematology and Blood Transfusion, Bingham University Teaching Hospital, Jos, Nigeria. Author
  • S. Awwalu Department of Haematology and Blood Transfusion, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria.  Author
  • A. D. Waziri Department of Haematology and Blood Transfusion, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria.  Author
  • A. Hassan Department of Haematology and Blood Transfusion, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria.  Author
  • A. A. Babadoko Department of Haematology and Blood Transfusion, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria.  Author
  • A. I.  Mamman Department of Haematology and Blood Transfusion, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria.  Author

Keywords:

hydroxyurea, soluble L-selectin, thrombospondin-1, steady state, sickle cell anaemia

Abstract

Background:  Sickle cell anaemia (SCA) is characterized by unpredictable episodes of vasoocclusion. Adhesion molecules, sickled red blood cells and inflammatory stimuli all contribute to these episodes. Hydroxyurea (HU) has proven to be effective in reducing the frequency  of  pain  episodes  in  SCA, notably via an Hb F dependent mechanism.  
 
Aims and Objectives:  The objectives of this study are to determine the relationship between HU therapy and serum levels of thrombospondin-1 and soluble L-selectin in patients with SCA; and assessed the relationship  between  the  use  of  HU  and clinical severity of the disease. 
 
Materials and Methods:  Sixty (60) patients with  SCA (30 HU exposed and  30  HU  naïve  patients  respectively),  all  in steady state, were recruited. They were administered structured questionnaire. Clinical determinants and laboratory tests, which include full blood count, soluble L-selectin and thrombospondin-1 were assessed. Clinical severity scores were also computed. Appropriate  statistical tools  were  utilised  for data analysis. 
 
Results:  Patients  with  SCA  exposed  to  HU  had  lower values of thrombospondin-1 and soluble Lselectin  compared  to  HU  naïve controls  (P  < 0.001; and P = 0.121 respectively). Most patients on HU had mild disease when compared to their HU naïve counterparts based on  clinical  severity  score  {29/30  (96.7%)  vs 18/30 (60.0%), χ2 = 13.918; df = 2; P = 0.001}. There  was  a  significant  correlation between thrombospondin-1  levels  and  clinical  severity score in both HU exposed and HU naïve patients with SCA (rho = 0.545; P < 0.001 and rho = 0.509; P = 0.002 respectively). Soluble Lselectin levels showed no significant correlation with clinical severity score in both groups (rho = 0.256; P = 0.086; and rho = 0.255; P = 0.173 respectively). 
 
Conclusion:  The usage of HU may significantly reduce levels  of  thrombospondin-1,  but  not  those  of soluble L-selectin in patients with SCA. 

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Published

2021-01-01

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How to Cite

Adebayo , A., Awwalu , S., Waziri , A. D., Hassan , A., Babadoko , A. A., & Mamman , A. I. (2021). Effect Of Hydroxyurea On Thrombospondin-1 And Soluble L-Selectin Levels Among Steady State Patients With Sickle Cell Anaemia . Nigerian Journal of Haematology, 5(1&amp;2), 22-27. https://njhaem.org.ng/index.php/home/article/view/55

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